Recombinant Anti-ErbB4 / HER4 (phospho Y1162) antibody [EP2270Y] (ab68478) (2024)

Overview

• Product name

  Anti-ErbB4 / HER4 (phospho Y1162) antibody [EP2270Y]
  See all ErbB4 / HER4 primary antibodies

• Description

  Rabbit monoclonal [EP2270Y] to ErbB4 / HER4 (phospho Y1162)

• Host species

  Rabbit

• Tested applications

  Suitable for: WB, IP, IHC-Pmore details
  Unsuitable for: Flow Cyt or ICC/IF

• Species reactivity

  Reacts with: Human
  

• Immunogen

  Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

• Positive control

  • WB: A431 treated with 100 ng/ml EGF.IHC-P: Human breast carcinoma tissue.IP: A431 cells.

• General notes

  This product has switched from a hybridoma to recombinant production method on 31st May 2023.

  This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free production

  For more information see here.

  Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

  See Also

  APC anti-human erbB3/HER-3 Antibody anti-erbB3 - 1B4C3Expression of c-erbB-2 Protein Detected in Adenocarcinoma Arising from Parotid Pleomorphic Adenoma - [Download PDF]

Properties

• Form

  Liquid

• Storage instructions

  Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

• Storage buffer

  pH: 7.20
  Preservative: 0.01% Sodium azide
  Constituents: 0.05% BSA, 40% Glycerol (glycerin, glycerine), 59% PBS

• Concentration information loading...

• Purity

  Protein A purified

• Clonality

  Monoclonal

• Clone number

  EP2270Y

• Isotype

  IgG

• Research areas

  • Signal Transduction
  • Protein Phosphorylation
  • Tyrosine Kinases
  • Receptor Tyrosine Kinases

  • Signal Transduction
  • Growth Factors/Hormones
  • EGF

  • Cancer
  • Growth factors
  • EGF

  • Cancer
  • Oncoproteins/suppressors
  • Oncoproteins
  • Growth factors

  • Cancer
  • Tumor biomarkers
  • Oncoproteins

  • Neuroscience
  • Development

Associated products

• Alternative Versions

  • Anti-ErbB4 / HER4 (phospho Y1162) antibody [EP2270Y] - BSA and Azide free (ab247386)

• Isotype control

  • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)

• Positive Controls

  • A-431 membrane extract lysate (ab30134)

• Recombinant Protein

  • Recombinant human ErbB4 / HER4 protein (ab155610)

Applications

Target

• Function

  Specifically binds and is activated by neuregulins, NRG-2, NRG-3, heparin-binding EGF-like growth factor, betacellulin and NTAK. Interaction with these factors induces cell differentiation. Not activated by EGF, TGF-A, and amphiregulin. The C-terminal fragment (CTF) of isoform JMA-A CYT-2 (containing E4ICD2) can stimulate transcription in the presence of YAP1. ERBB4 intracellular domain is involved in the regulation of cell growth. Conflicting reports are likely due at least in part to the opposing effects of the isoform-specific and nuclear-translocated ERBB4 intracellular domains (E4ICD1 and E4ICD2). Overexpression studies in epithelium show growth inhibition using E4ICD1 and increased proliferation using E4ICD2. E4ICD2 has greater in vitro kinase activity than E4ICD1. The kinase activity is required for the nuclear translocation of E4ICD2.

• Tissue specificity

  Expressed at highest levels in brain, heart, kidney, in addition to skeletal muscle, parathyroid, cerebellum, pituitary, spleen, testis and breast. Lower levels in thymus, lung, salivary gland, and pancreas. Isoform JM-A CYT-1 and isoform JM-B CYT-1 are expressed in cerebellum, but only the isoform JM-B is expressed in the heart.

• Sequence similarities

  Belongs to the protein kinase superfamily. Tyr protein kinase family. EGF receptor subfamily.
  Contains 1 protein kinase domain.

• Post-translational
  modifications

  Isoform JM-A CYT-1 and isoform JM-A CYT-2 but not isoform JM-B CYT-1 and isoform JM-B CYT-2 are processed by ADAM17. Proteolytic processing in response to ligand or 12-O-tetradecanoylphorbol-13-acetate stimulation results in the production of 120 kDa soluble receptor forms and intermediate membrane-anchored 80 kDa fragments (m80HER4), which are further processed by a presenilin-dependent gamma-secretase to release the respective cytoplasmic intracellular domain E4ICD (either E4ICD1/s80Cyt1 or E4ICD2/s80Cyt2). Membrane-anchored 80 kDa fragments of the processed isoform JM-A CYT-1 are more readily degraded by the proteasome than fragments of isoform JM-A CYT-2 suggesting a prevalence of E4ICD2 over E4ICD1.
  Ligand-binding increases phosphorylation on tyrosine residues. Isoform JM-A CYT-2 is constitutively phosphorylated on tyrosine residues in a ligand-independent manner. E4ICD2 but not E4ICD1 is phosphorylated on tyrosine residues.
  Ubiquitinated. The ERBB4 intracellular domain is ubiquitinated and targeted to proteosomal degradation during mitosis mediated by the APC/C complex. Isoform JM-A CYT-1 and isoform JM-B CYT-1 are ubiquitinated by WWP1. The ERBB4 intracellular domain (E4ICD1) is ubiquitinated, and this involves NEDD4.

• Cellular localization

  Membrane and Nucleus. Following proteolytical processing E4ICD (E4ICD1 or E4ICD2 generated from the respective isoforms) is translocated to the nucleus. Significantly more E4ICD2 than E4ICD1 is found in the nucleus. E4ICD2 colocalizes with YAP1 in the nucleus.

• Information by UniProt

• Database links

  • Entrez Gene: 2066 Human
  • Omim: 600543 Human
  • SwissProt: Q15303 Human
  • Unigene: 390729 Human

• Alternative names

  • 4ICD antibody
  • ALS19 antibody
  • Avian erythroblastic leukemia viral oncogene hom*olog 4 antibody

  see all

Images

Protocols

Datasheets and documents

References (3)